Research
ULK1 knockout increases recombinant transcript abundance and improves recombinant membrane protein expression
Cell Systems · By Alice Karlsson, Melanie Dannemeyer, Jenny Gunnarsson, Niklas Berndt Thalén, Jennifer Hicks, Magnus Lundqvist, Anna Backmark, Susanna Engberg, Carina Raynoschek, Anna-Luisa Volk, Ramy Elgendy, Michael J. Munson, Magdalena Malm, Robert G. Roth & Johan Rockberg ·
The Brief
- The authors present an ULK1 knockout Expi293 cell line, identified through transcriptomic analyses as suitable for membrane protein production.
- Production of three model membrane proteins in the knockout line reached up to a doubling in titer.
- Additional transcriptomics confirm ULK1 as a factor affecting recombinant transcript abundance.
Why it matters
Membrane proteins are difficult to produce in quantity, so a cell line that raises recombinant yields could make structural and functional studies of these proteins more practical.
Who’s affected
- researchers producing recombinant membrane proteins
- labs using Expi293 cells for protein production
- #ulk1
- #expi293
- #membrane proteins
- #recombinant protein expression
- #cell line engineering
The authors present an ULK1 knockout Expi293 cell line that, through transcriptomic analyses, indicated suitability for membrane protein production. Production of three model membrane proteins generated up to a doubling in titer. Additional transcriptomics confirm ULK1 as a…
Timeline
Study published in Cell Systems
cell.com
ULK1 knockout increases recombinant transcript abundance and improves recombinant membrane protein expression