Research
Promoter-proximal pausing enables transcriptional buffering of RNA polymerase II availability
Molecular Cell · By Anouk M. Olthof, Nanna Bach Poulsen, Melvin J. Noe Gonzalez, Nicolas Nieto Moreno, Theodora Bautu, Simona Cugusi & Jesper Q. Svejstrup ·
The Brief
- Olthof et al. titrated cellular RPB1 levels and found that transcription is surprisingly sensitive to RNA polymerase II dosage.
- Genes differ widely in how sensitive they are to RNA polymerase II dosage.
- Genes involved in transcriptional maintenance are typically dosage insensitive.
Why it matters
The study describes how cells can buffer changes in RNA polymerase II availability, which bears on how gene expression is regulated across genes with different sensitivities.
Who’s affected
- molecular biologists studying gene transcription
- #transcription
- #rna polymerase ii
- #promoter-proximal pausing
- #gene expression
- #rpb1
Through titration of cellular RPB1 levels, Olthof et al. show that transcription is surprisingly sensitive to RNAPII dosage, but that genes vary widely in their sensitivity. Genes involved in transcriptional maintenance are typically dosage insensitive. Expression of these genes…
Timeline
Study published in Molecular Cell
cell.com
Promoter-proximal pausing enables transcriptional buffering of RNA polymerase II availability