Research
A split RNA-guided Cas9 system for PAM-independent and cis-cleavage-free nucleic acid detection
Nature ·
The Brief
- A paper titled "A split RNA-guided Cas9 system for PAM-independent and cis-cleavage-free nucleic acid detection" is listed in nature.com's biological sciences feed.
- The title describes a split RNA-guided Cas9 system for detecting nucleic acids that does not depend on a PAM sequence and avoids cis-cleavage.
- No summary or further details were provided in the sources.
Why it matters
If the approach works as the title describes, it could make Cas9-based nucleic acid detection less constrained by PAM requirements and by cis-cleavage, which matters for researchers designing CRISPR-based detection assays.
Who’s affected
- researchers in CRISPR-based diagnostics and molecular biology
- #crispr
- #cas9
- #nucleic-acid-detection
- #diagnostics
- #molecular-biology
Timeline
Paper listed in nature.com biological sciences feed (Nature Communications)
Biological sciences : nature.com subject feeds
A split RNA-guided Cas9 system for PAM-independent and cis-cleavage-free nucleic acid detection