Research
A sequence-encoded promoter-proximal super pause stabilizes an offline RNA polymerase II state
Molecular Cell · By Roberto J. Vazquez Nunez, Serena Kesha & Seychelle M. Vos ·
The Brief
- Vazquez Nunez et al. developed GATO-seq, a cell-free genomics method that directly measures RNA polymerase II elongation dynamics.
- They identified a "super pause" DNA sequence near gene start sites that alone potently pauses RNA polymerase II.
- The sequence traps the enzyme in a previously undescribed state, according to the source.
Why it matters
It shows that DNA sequence near gene start sites can by itself hold RNA polymerase II in a paused state, which bears on how gene expression is controlled.
Who’s affected
- molecular biologists studying transcription
- geneticists studying gene regulation
- #transcription
- #rna polymerase ii
- #promoter-proximal pausing
- #gene regulation
- #genomics
Vazquez Nunez et al. develop GATO-seq, a cell-free genomics method to directly measure RNA polymerase II elongation dynamics. They identify a “super pause” DNA sequence near gene start sites that alone potently pauses RNA polymerase II, trapping it in a yet undescribed…
Timeline
Study published in Molecular Cell
cell.com
A sequence-encoded promoter-proximal super pause stabilizes an offline RNA polymerase II state