# A split RNA-guided Cas9 system for PAM-independent and cis-cleavage-free nucleic acid detection

Source: [Biological sciences : nature.com subject feeds](https://www.nature.com/articles/s41467-026-79722-z) · 2026-11-14 · Life
Page: https://themorning.science/story/a-split-rna-guided-cas9-system-for-pam-independent-and-cis-cleavage-free-nucleic-ed6eebc3ed609ac5
Kind: research · Impact: low

## In brief

- A paper titled "A split RNA-guided Cas9 system for PAM-independent and cis-cleavage-free nucleic acid detection" is listed in nature.com's biological sciences feed.
- The title describes a split RNA-guided Cas9 system for detecting nucleic acids that does not depend on a PAM sequence and avoids cis-cleavage.
- No summary or further details were provided in the sources.

## Why it matters

If the approach works as the title describes, it could make Cas9-based nucleic acid detection less constrained by PAM requirements and by cis-cleavage, which matters for researchers designing CRISPR-based detection assays.

## Timeline

- 2026-11-14: Paper listed in nature.com biological sciences feed (Nature Communications)

## Coverage

- [A split RNA-guided Cas9 system for PAM-independent and cis-cleavage-free nucleic acid detection](https://www.nature.com/articles/s41467-026-79722-z) — Biological sciences : nature.com subject feeds, 2026-11-14

Tags: crispr, cas9, nucleic-acid-detection, diagnostics, molecular-biology

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Brief generated by AI (claude-haiku-5-5) from the reporting listed under Coverage. Headlines belong to their publishers. The Morning Science – https://themorning.science